Wiki:Sandbox

From Helminthic Therapy wiki
Revision as of 22:56, 14 October 2021 by John (talk | contribs) (→‎The problem with clinical trials: Removed this entire section from this location for addition to the Research page.)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search

Cleaning larvae that are to be shared with others[edit]

Given that hookworms shed a layer of skin before entering a host, it is very unlikely that hookworms would transmit infectious organisms to a host, and a literature search yielded no record of pathogen transmission between hookworm and host. [1] However, the possibility of pathogen transmission between hookworm hosts as a result of inoculation by one individual with larvae grown from the faeces of another has not been ruled out unequivocally. This is why the commercial providers of NA periodically test their reservoir donors for a range of communicable diseases such as HIV and hepatitis, always use an antimicrobial wash to clean the larvae they supply, and then ship them in a weak antibiotic solution. These precautionary measures are as far as the hookworm providers can go to obviate the risk of pathogen transmission, given that helminths are living organisms, and would be killed by any attempt to completely sterilise them.

Hookworm larvae do not need to be cleaned if they have been grown at home for use by the same individual who provided the stool sample for their incubation. However, if that individual wishes to share the larvae they have grown, these can be cleaned using one of the following methods.

Bleach treatment

Collect the brown water from the bottom of the container used for incubation and leave this in a champagne glass for 24 hours. Then add one or two drops of bleach to one litre of water and use some of the resulting bleach solution to almost fill a second champagne glass. The next day, use a pipette to carefully draw up the worms that have settled to the bottom of the champagne glass containing the brown water. Avoid squeezing the pipette once it is in the water because this would create bubbles that would disturb any sediment and distribute the larvae. Instead, slightly squeeze the bulb of the pipette before it enters the water, and hold this position carefully until the pipette reaches the bottom of the glass, where it can be gently released to collect the larvae. Then add the larvae collected to the bleach solution in the second champagne glass. Leave the larvae in the bleach solution for a further 24 hours. The following day, use a pipette to draw up the larvae from the bottom of the bleach solution and transfer them to a microscope slide for counting in preparation for inoculation, or add them to an eppendorf tube containing fresh water (distilled, filtered or bottled water, or dechlorinated tap water) for supply to someone else. [2]

Iodine treatment

Add one drop of commercially available 5% Lugol’s iodine to 5 ml of water (distilled, filtered or bottled water, or dechlorinated tap water) to create a 0.02% iodine solution. Then add 1 ml of this solution to 1 ml of water containing the larvae to be cleaned. After the larvae have spent 20 minutes in this solution, the iodine should be neutralised by adding a pinch of ascorbic acid (vitamin C) powder to the solution. Neutralisation of the iodine is confirmed when sufficient ascorbic acid has been sprinkled into the water to make this completely clear.

Larvae that have been cleaned by any method will have a shorter shelf life than those that have not been cleaned.


                                            --------------------------------

Options for text boxes - PLEASE DO NOT DELETE[edit]

Blue background / blue border / round corners
Blue background / orange border / round corners
Blue background / blue border / squared corners
Blue background / orange border / squared corners
Pink background / orange border / round corners
Pink background / blue border / round corners
Pink background / orange border / squared corners
Pink background / blue border / squared corners
Buff background / orange border / large round corners - Home page
Uncoloured background / orange border / large round corners - Providers page