Difference between revisions of "Helminth inoculation"

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After being pipetted onto a bandage/dressing, the hookworm larvae should be applied to a hairless, convex area of skin to ensure good skin contact, as demonstrated in the following instructional video.
 
After being pipetted onto a bandage/dressing, the hookworm larvae should be applied to a hairless, convex area of skin to ensure good skin contact, as demonstrated in the following instructional video.
* [https://docs.google.com/file/d/0B4BIMpM29I7zaDNxTW9Ka2dadHc/edit Hookworm inoculation video 1]
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* [https://docs.google.com/file/d/0B4BIMpM29I7zaDNxTW9Ka2dadHc/edit Hookworm inoculation using larvae suspended in liquid]
  
 
There can be stragglers trapped in water droplets in the vial after the contents have been pipetted onto a bandage/dressing, so at least one rinse should be carried out. Even a second rinse can capture the odd straggler. [https://www.facebook.com/groups/htsupport/permalink/1669376486451633/]
 
There can be stragglers trapped in water droplets in the vial after the contents have been pipetted onto a bandage/dressing, so at least one rinse should be carried out. Even a second rinse can capture the odd straggler. [https://www.facebook.com/groups/htsupport/permalink/1669376486451633/]
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Hookworm larvae from some providers may be supplied pre-loaded onto a small damp cotton patch, which is then placed onto a larger bandage/dressing before being applied to the skin, as demonstrated in the following video.   
 
Hookworm larvae from some providers may be supplied pre-loaded onto a small damp cotton patch, which is then placed onto a larger bandage/dressing before being applied to the skin, as demonstrated in the following video.   
* [https://www.youtube.com/watch?v=hm32xL2e6CA&feature=youtu.be&app=desktop Hookworm inoculation video 2]
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* [https://www.youtube.com/watch?v=hm32xL2e6CA&feature=youtu.be&app=desktop Hookworm inoculation using a pre-loaded patch]
  
 
The bandage/dressing should be left in place for a minimum of four hours and, ideally, for twelve hours.  
 
The bandage/dressing should be left in place for a minimum of four hours and, ideally, for twelve hours.  

Revision as of 16:48, 26 May 2018

Inoculation with NAthe human hookworm, Necator americanus

NAthe human hookworm, Necator americanus larvaeThe active immature form of an insect, or an animal such as a helminth, which develops from an egg and eventually transforms again into its adult state. should be used as soon as they are available. They can survive for as long as 3-5 months if kept at a favourable temperature (70ºF/21ºC), although their longevity is also dependent on the temperature at which their were incubated, with larvaeThe active immature form of an insect, or an animal such as a helminth, which develops from an egg and eventually transforms again into its adult state. grown at a lower temperature surviving for longer. Since they do not feed at this stage in their development, the larvaeThe active immature form of an insect, or an animal such as a helminth, which develops from an egg and eventually transforms again into its adult state. are reliant on their fat stores, which obviously diminish over time, rendering them progressively weaker.

After being pipetted onto a bandage/dressing, the hookwormA helminth that lives in the small intestine. Necator americanus (NA) is the only hookworm species used in helminthic therapy. Its microscopic larvae are applied periodically to the skin. larvaeThe active immature form of an insect, or an animal such as a helminth, which develops from an egg and eventually transforms again into its adult state. should be applied to a hairless, convex area of skin to ensure good skin contact, as demonstrated in the following instructional video.

There can be stragglers trapped in water droplets in the vial after the contents have been pipetted onto a bandage/dressing, so at least one rinse should be carried out. Even a second rinse can capture the odd straggler. [1]

If inoculating with home-grown larvaeThe active immature form of an insect, or an animal such as a helminth, which develops from an egg and eventually transforms again into its adult state., use a bandage/dressing with a flat central pad in preference to the quilted type. A Band-Aid dressing with a ⚠️"Quilt-Aid Comfort Pad" has been reported to have prevented successful inoculationThe introduction of an infectious agent into an organism. [http://helminthictherapywiki.org/wiki/index.php/Helminth_inoculation Helminth inoculation] in one case. [2]

If a ready-made bandage/dressing is not available, an effective substitute can be made from a piece of flat paper towel stuck to a piece of masking tape. [3]

HookwormA helminth that lives in the small intestine. Necator americanus (NA) is the only hookworm species used in helminthic therapy. Its microscopic larvae are applied periodically to the skin. larvaeThe active immature form of an insect, or an animal such as a helminth, which develops from an egg and eventually transforms again into its adult state. from some providers may be supplied pre-loaded onto a small damp cotton patch, which is then placed onto a larger bandage/dressing before being applied to the skin, as demonstrated in the following video.

The bandage/dressing should be left in place for a minimum of four hours and, ideally, for twelve hours.

When the bandage is removed, this may reveal the beginnings of a rash. For more about this, see the following page.

For tips on dealing with the inoculationThe introduction of an infectious agent into an organism. [http://helminthictherapywiki.org/wiki/index.php/Helminth_inoculation Helminth inoculation] rash, see this page.

Best time of day to inoculate with NAthe human hookworm, Necator americanus

Different self-treaters prefer different times.

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I get a very aggressive itch, so I always inoculate in the morning. This way, I can distract myself during the day. I take the bandage/dressing off before I retire for the night, and take my hair dryer to bed with me. [4]
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I like to inoculate at night because I sleep through the itch! I hair dryer in the morning. [5]

Body sites used for hookwormA helminth that lives in the small intestine. Necator americanus (NA) is the only hookworm species used in helminthic therapy. Its microscopic larvae are applied periodically to the skin. inoculationThe introduction of an infectious agent into an organism. [http://helminthictherapywiki.org/wiki/index.php/Helminth_inoculation Helminth inoculation]

The inside of the forearm or bicep is a good choice because it is convenient to access in order to treat the rash with creams, etc., but inoculationThe introduction of an infectious agent into an organism. [http://helminthictherapywiki.org/wiki/index.php/Helminth_inoculation Helminth inoculation] too close to the armpit can cause lymph nodes there to swell, and inoculationThe introduction of an infectious agent into an organism. [http://helminthictherapywiki.org/wiki/index.php/Helminth_inoculation Helminth inoculation] high on the inner thigh can raise lymph nodes in the groin.

Sites that have been used include the following.

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I use somewhere on my legs because it's easier to hide under clothes. I always get an awfully itchy itch -- but slightly less when using the upper parts of the leg. [6]
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I like to do ankles. [7]
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It always itches badly, but say the most sensitive places such as inner lower arm and inner calf (not to mention the hollow of the knee :-o ) are "10" on the itching scale, then the middle of the thigh, or slightly above the knee, is "7" or "8"... [8]
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Since it's summer, I think I'm going for left thigh or hip this time. [9]
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I did my second dose on my thigh and that worked fine. [10]
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I've done my thigh but much prefer my arm as the itch seems more managable. [11]
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I started inoculating high on my shoulder because I sleep on my side, and that part is always squished against and rubbing around on the bed, and plus not very sensitive, so the itch is quite bearable. [12]
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I prefer the inner left forearm because I’m right handed and this makes applying anti-itch cream easier. [13]
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I always use the place on my inner arm halfway between my elbow and wrist, because it's flat and smooth... The forearm can easily be covered with a light sleeve but also quickly accessed for airing out or changing dressing. [14]
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Calf. [15]
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I put them somewhere that isn't hard to scratch. Down on my calf I'd be bent over all day long. [16]
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I always do my stomach. [17]

Many hookwormA helminth that lives in the small intestine. Necator americanus (NA) is the only hookworm species used in helminthic therapy. Its microscopic larvae are applied periodically to the skin. hosts have found that repeatedly using the same site results in a more robust skin reaction, and that varying the site effectively reduces both the rash and the itching.

Oral inoculationThe introduction of an infectious agent into an organism. [http://helminthictherapywiki.org/wiki/index.php/Helminth_inoculation Helminth inoculation] with NAthe human hookworm, Necator americanus is not recommended

There are several reasons why it is not recommended to swallow NAthe human hookworm, Necator americanus larvaeThe active immature form of an insect, or an animal such as a helminth, which develops from an egg and eventually transforms again into its adult state..

1. Oral inoculationThe introduction of an infectious agent into an organism. [http://helminthictherapywiki.org/wiki/index.php/Helminth_inoculation Helminth inoculation] has been shown to be effective with some hookwormA helminth that lives in the small intestine. Necator americanus (NA) is the only hookworm species used in helminthic therapy. Its microscopic larvae are applied periodically to the skin. species, such as the dog hookwormA helminth that lives in the small intestine. Necator americanus (NA) is the only hookworm species used in helminthic therapy. Its microscopic larvae are applied periodically to the skin., Ancylostoma caninum, [18] and the human hookwormA helminth that lives in the small intestine. Necator americanus (NA) is the only hookworm species used in helminthic therapy. Its microscopic larvae are applied periodically to the skin., Ancylostoma duodenaleA species of roundworm, aka the Old World hookworm, that is "not" used in helminthic therapy. [https://en.wikipedia.org/wiki/Ancylostoma_duodenale Wikipedia:Ancylostoma duodenale], [19] but oral inoculationThe introduction of an infectious agent into an organism. [http://helminthictherapywiki.org/wiki/index.php/Helminth_inoculation Helminth inoculation] with Necator americanusThe species of human hookworm used in helminthic therapy. Its microscopic larvae are applied periodically to the skin. has been found to be ineffective.

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N. americanus may require a period of growth in the lungs, which is not required by A. duodenale. This deduction is based on the observation that development of N. americanus following oral infection rarely succeeds and, even then, very few worms develop… the apparent inability of N. americanus to do this is thought to be attributable to an obligate pneumonic phase in its life history. [20]

2. HookwormsA helminth that lives in the small intestine. Necator americanus (NA) is the only hookworm species used in helminthic therapy. Its microscopic larvae are applied periodically to the skin. have probably taken the same route throughout most of their long co-evolution with mankind, so they are likely to try to follow the same pathway irrespective of where they actually enter the body. Therefore, unless they are encapsulated, larvaeThe active immature form of an insect, or an animal such as a helminth, which develops from an egg and eventually transforms again into its adult state. that are swallowed may still be looking for skin, and might mistake the lining of the mouth or throat for external skin and enter via these surfaces. Nagahana, et al., have reported that N. americanus L3 larvaeThe active immature form of an insect, or an animal such as a helminth, which develops from an egg and eventually transforms again into its adult state. will invade the buccal epithelium if they enter through the mouth. (Nagahana M, et al. Experimental studies on the oral infection of Necator americanusThe species of human hookworm used in helminthic therapy. Its microscopic larvae are applied periodically to the skin.. III. Experimental infection of three cases of human beings with Necator americanusThe species of human hookworm used in helminthic therapy. Its microscopic larvae are applied periodically to the skin. larvaeThe active immature form of an insect, or an animal such as a helminth, which develops from an egg and eventually transforms again into its adult state. through the mucous membrane of the mouth. Japanese Journal of Parasitology. 1963;12:162–167.) Since some people experience significant swelling at the inoculationThe introduction of an infectious agent into an organism. [http://helminthictherapywiki.org/wiki/index.php/Helminth_inoculation Helminth inoculation] site, there is a potential for blockage of the throat if larvaeThe active immature form of an insect, or an animal such as a helminth, which develops from an egg and eventually transforms again into its adult state. were swallowed.

3. L3 hookwormA helminth that lives in the small intestine. Necator americanus (NA) is the only hookworm species used in helminthic therapy. Its microscopic larvae are applied periodically to the skin. larvaeThe active immature form of an insect, or an animal such as a helminth, which develops from an egg and eventually transforms again into its adult state. are particularly susceptible to acid, so there must be some developmental change that occurs during the few days that they normally take to travel from the skin to the stomach which enables them to survive the harsh, acidic gastric environment. Hotez and colleagues have reported that, following entry into the host via the skin, L3 larvaeThe active immature form of an insect, or an animal such as a helminth, which develops from an egg and eventually transforms again into its adult state. receive a signal present in mammalian blood and tissue that causes them to resume development and secrete bioactive polypeptides. [21] [22] Larvae that are swallowed will obviously not experience this trigger.

4. Oral inoculationThe introduction of an infectious agent into an organism. [http://helminthictherapywiki.org/wiki/index.php/Helminth_inoculation Helminth inoculation] does not provide the visible confirmation of dose viability that is common with successful percutaneous inoculationThe introduction of an infectious agent into an organism. [http://helminthictherapywiki.org/wiki/index.php/Helminth_inoculation Helminth inoculation]. So, unless the worms have been cultured by the person inoculating, or they have a microscope with which to check the viability of doses received from other sources, they might unknowingly swallow a dose of dead worms, which could delay the progress of their treatment.

Possible side effects after inoculationThe introduction of an infectious agent into an organism. [http://helminthictherapywiki.org/wiki/index.php/Helminth_inoculation Helminth inoculation] with NAthe human hookworm, Necator americanus

See the following pages.

Inoculation with TTOthe ova (eggs) of the human whipworm, Trichuris trichiura

TTOthe ova (eggs) of the human whipworm, Trichuris trichiura are taken orally in a drink.

Possible side effects after inoculationThe introduction of an infectious agent into an organism. [http://helminthictherapywiki.org/wiki/index.php/Helminth_inoculation Helminth inoculation] with TTOthe ova (eggs) of the human whipworm, Trichuris trichiura

See the following page section.

Inoculation with TSOthe ova (eggs) of the porcine (pig) whipworm, Trichuris suis

TSOthe ova (eggs) of the porcine (pig) whipworm, Trichuris suis are taken orally in a drink.

Possible side effects after inoculationThe introduction of an infectious agent into an organism. [http://helminthictherapywiki.org/wiki/index.php/Helminth_inoculation Helminth inoculation] with TSOthe ova (eggs) of the porcine (pig) whipworm, Trichuris suis

See the following page section.

Inoculation with HDCHymenolepis diminuta cysticercoids (Hi-men-o-lep'is dim-a-nu-ta sis-ti-sur-koid) - the larval cysts of the rat tapeworm, Hymenolepis diminuta

HDCHymenolepis diminuta cysticercoids (Hi-men-o-lep'is dim-a-nu-ta sis-ti-sur-koid) - the larval cysts of the rat tapeworm, Hymenolepis diminuta are taken orally in a drink, preferably something containing fat to help promote the growth of the cysticerciThe larval (immature) stage of a tapeworm.. Milk will work well, or a single fish oil capsule can be taken with any other liquid.

Possible side effects after inoculationThe introduction of an infectious agent into an organism. [http://helminthictherapywiki.org/wiki/index.php/Helminth_inoculation Helminth inoculation] with HDCHymenolepis diminuta cysticercoids (Hi-men-o-lep'is dim-a-nu-ta sis-ti-sur-koid) - the larval cysts of the rat tapeworm, Hymenolepis diminuta

See the following page and page section.