The first inoculation should be with a single species
When two species of worm are introduced together by someone who has not previously hosted helminths, or not hosted for some time, the initial immune response may be excessive and the risk of side effects greatly increased.
If helminthic therapy is commenced with a single species of worm, and if initial dosing is conservative, any side effects that appear should be mild and transient.
There may be an advantage in adding a second species eventually, but only once the first is well established.
Inoculation is safe, even when the recipient has a cold
Research suggests that helminths may boost humoral immunity, so inoculation with appropriately sized doses of therapeutic helminths might actually be helpful if taken while suffering from a cold or the flu. However, chronic infections may be exacerbated temporarily by inoculation, especially the first time that a helminth is introduced.
Inoculation with NA
NA larvae are applied to the skin on a bandage/dressing and should be used as soon as they are available.
They can survive for as long as 3-5 months if kept at a favourable temperature (70ºF/21ºC), although their longevity is also dependent on the temperature at which they were incubated, with larvae grown at a lower temperature surviving for longer. Since they do not feed at this stage in their development, the larvae are reliant on their fat stores, which obviously diminish over time, rendering them progressively weaker. For more detail, see the following page.
After being pipetted onto a bandage/dressing, the hookworm larvae should be applied to a hairless, convex area of skin to ensure good skin contact, as demonstrated in the following instructional video.
There can be stragglers trapped in water droplets in the vial after the contents have been pipetted onto a bandage/dressing, so at least one rinse should be carried out. Some providers supply a second vial of distilled water for this purpose. Even a second rinse can capture the odd straggler. 
If inoculating with home-grown larvae, use a bandage/dressing with a flat central pad in preference to the quilted type. A Band-Aid dressing with a ⚠️"Quilt-Aid Comfort Pad" has been reported to have prevented successful inoculation in one case. 
If a ready-made bandage/dressing is not available, an effective substitute can be made by using a makeup pad, or by cutting the required size from a large lint pad and securing this to the skin with medical tape. . Alternatively, a piece of toilet paper, or flat paper towel, stuck to a piece of masking/painter's tape will work.  
Some hookworm suppliers have experimented with pre-loading the larvae onto a small damp cotton patch, which needs to be placed onto a larger bandage/dressing after receipt, before being applied to the skin. So far, this method has proved to be less successful than the traditional method requiring the self-treater to pipette the larvae from an eppendorf tube onto a bandage/dressing, but, for those who do receive a pre-loaded patch, this can be applied as demonstrated in the following video.
After the bandage/dressing has been applied, the larvae will detect the presence of skin   and, responding in particular to warmth,  will swing into action, often producing an itch within just a few minutes. Occasionally, the itch may be delayed for up to several hours,  or even until the next day. 
The bandage/dressing should be left in place for a minimum of four hours and, ideally, for twelve hours. If you need to take a shower before the bandage/dressing is due to come off, cut a piece from a plastic bag and tape this over the area to keep it dry.  Duct tape or electrical insulation tape are ideal for this purpose because they are waterproof.
When the bandage is removed, this may reveal the beginnings of a rash. For more about this and how to treat the itch, see the following page.
Best time of day to inoculate with NA
Different self-treaters prefer to inoculate at different times during the day.
Body sites used for hookworm inoculation
The inside of the forearm or bicep is a good choice because it is convenient to access in order to treat the rash with creams or apply hot air from a hair dryer, but inoculation too close to the armpit can cause lymph nodes there to swell. Inoculation high on the inner thigh - and even occasionally on the outer thigh  - can cause painfully raised lymph nodes in the groin, which can be uncomfortable when walking. 
Many hookworm hosts have found that repeatedly using the same site results in a more robust skin reaction, and that varying the site effectively reduces both the rash and the itching.
Sites that have been used include the following, starting with the feet and moving up to the shoulder.
- However, someone else who tried the underside of their foot has said, "Never again"! 
One site that should be avoided is the thumb!
Oral inoculation with NA is not recommended
There are several reasons why it is not recommended to swallow NA larvae.
1. While oral inoculation has been shown to be effective with some hookworm species, such as the dog hookworm, Ancylostoma caninum,  and the human hookworm, Ancylostoma duodenale,  oral inoculation with Necator americanus has been found to be ineffective, possibly because passage through the lungs is omitted.
2. L3 hookworm larvae are particularly susceptible to acid, so there must be some developmental change that occurs during the few days that they normally take to travel from the skin to the stomach which enables them to survive the harsh, acidic gastric environment. Hotez and colleagues have reported that, following entry into the host via the skin, L3 larvae receive a signal present in mammalian blood and tissue that causes them to resume development and secrete bioactive polypeptides.   Larvae that are swallowed will obviously not experience this trigger.
3. Hookworms have probably taken the same route throughout most of their long co-evolution with mankind, so they are likely to try to follow the same pathway irrespective of where they actually enter the body. Therefore, unless they are encapsulated, larvae that are swallowed may still be looking for skin, and might mistake the lining of the mouth or throat for external skin and enter via these surfaces. Nagahana, et al., have reported that N. americanus L3 larvae will invade the buccal epithelium if they enter through the mouth. (Nagahana M, et al. Experimental studies on the oral infection of Necator americanus. III. Experimental infection of three cases of human beings with Necator americanus larvae through the mucous membrane of the mouth. Japanese Journal of Parasitology. 1963;12:162–167.) Since some people experience significant swelling at the inoculation site, there is a potential for blockage of the throat if larvae were swallowed.
4. Oral inoculation does not provide the visible confirmation of dose viability that is common with successful percutaneous inoculation. So, unless the worms have been cultured by the person inoculating, or they have a microscope with which to check the viability of doses received from other sources, they might unknowingly swallow a dose of dead worms, which could delay the progress of their treatment.
Possible side effects after inoculation with NA
See the following pages.
Inoculation with TTO
TTO are taken orally in a drink.
Possible side effects after inoculation with TTO
See the following page section.
Inoculation with TSO
TSO are taken orally in a drink.
Possible side effects after inoculation with TSO
See the following page section.
Inoculation with HDC
HDC are taken orally in a drink, preferably something containing fat to help promote the growth of the cysticerci. Milk will work well, or a single fish oil capsule can be taken with any other liquid.
Possible side effects after inoculation with HDC
See the following page section.